Current Issue : October - December Volume : 2015 Issue Number : 4 Articles : 7 Articles
Indian traditional system of medicine prescribes herbal remedies for treatment of diseases including diabetes. In recent years, plants are being successfully tried in variety of pathophysiological states. The present investigation, evaluated antidiabetic effect of hydro alcoholic extract of Hippophae rhamnoides L. seeds (HAHRS) in alloxan-induced diabetic rats. HAHRS (286 and 667 mg/kg) and glibenclamide (1 mg/kg) treatment was given to diabetic rats for 21 days and at the end of experimental period, serum glucose, cholesterol, HDL (High Density Lipoprotein), liver glycogen and glucose-6-phosphatase activities estimations were carried out. Statistical significance was assessed using one-way analysis of variance (ANOVA) followed by tukey comparison test. Results showed that because of alloxan treatment, serum level glucose, cholesterol and glucose-6-phosphatase activity increased, while HDL and liver glycogen decrease significantly which could be effectively and significantly blunted by HAHRS (667 mg/kg) and glibenclamide. This finding suggest that significant antidiabetic activity of HAHRS could be result of synergistic/potentiative anti-oxidant action of its medicinal phytoconstituents such as flavonoids, vitamin and carotenoids, since one of proposed mechanisms of diabetic effect of alloxan is through generation of free radicals and diverse array of active principles which are able to target multiple mechanisms involved in pathophysiology of diabetes....
The present study shows the effect of administration of LSFP for sixty days on serum estrogen level of female Wistar rats received 300, 500 and 1000 mg kg-1 body weight of the LSFP. Female Wistar rats (180–200 g) were divided into four groups. Grp I: control, Grp II: low dose (300 mg/kg), Grp III: medium dose (500 mg/kg.) Grp IV: higher dose (1000 mg/kg). Prescribed doses of LSFP suspended in gum acacia (2%) were administered orally for 60 days. On day 60th blood was withdrawn by retro-orbital puncture for estimation of serum estrogen hormone level. Control group was compared with higher dose treatment groups. Histology of vital organs was performed. Significant increase in serum Estrogen hormone levels observed at the doses 1000 mg/kg of LSFP, while no significant differences were found at 300 mg/kg and 500 mg/kg doses of LSFP when compared to control group rats. While significant increase in ovarian and uterine weights were observed at the doses 500 mg/kg and 1000 mg/kg of LSFP and no changes were observed at the doses 300 mg/kg, when compared to control group rats. Significant increase in serum estrogen hormone level and ovarian and uterine weights. The phytochemical analysis shows presence of major chemical constituents as flavonoids, phytosterols (β-sitosterol, fucosterol, campesterol), alkaloids, 5-alpha-reductase enzyme inhibitors (oleic acid, Linoleic acid, palmitic acid and steric acid). On the basis of above evidences it may possible that the presence of phytosterols, 5-alpha-reductase enzyme inhibitors may be responsible for the observed results....
The present investigation was carried out to evaluate the aphrodisiac potential of the hydroalcoholic extract of whole plant of Argemone mexicana Linn. (HEAM) in rats. Male wistar albino rats were divided into three groups. Rats were orally treated with 1) Control group: distilled water; 2) HEAM 200 mg/kg/day and 3) HEAM 400 mg/kg/day; for 21 days and on 11th and 21st day, 01 hour after the respective treatments, each male rat was tested for their sexual behavior for the period of 30 minutes wherein 10 sexually experienced adult ovariectomised female rats (brought into estrous phase) were used as copulatory partners. During 30 minutes test period, various parameters of male rats sexual behavior like mount latency (ML), intromission latency (IL), ejaculation latency (EL), post ejaculatory interval (PEI), mount frequency (MF), intromission frequency (IF) and ejaculation frequency (EF) were recorded. HEAM enhanced sexual arousal, libido (sexual desire) and sexual vigor or sexual performance reflecting the aphrodisiac potential of HEAM. The results suggested the possible use of hydroalcoholic extract of whole plant of Argemone mexicana Linn. in boosting the sexual activity and libido in males....
In the present study defending potential of three indigenous plants assessed and compared using sperm abnormality nassay. Lagenaria siceraria, Desmodium gangeticum and Leucas aspera were selected on the basis of reported cytoprotective and antioxidant activity. Activity was evaluated using sperm abnormality assay. The animals were divided into four groups, four animals per group. The group distribution was, negative control, positive control (cyclophosphamide treated) and two treatment control group. Sperm abnormality was induced by administering cyclophosphamide (20 mg/kg, i.p) 24 hrs prior to sacrifice animals. Parameters selected are hook cut, banana shaped, amorphous shaped, double tailed and folded on them to execute sperm abnormality. The methanolic extract of selected indigenous plants had shown significant reduction in the total number of sperm abnormality. The results obtained by these experiments, strongly suggesting that Lagenaria siceraria and Desmodium gangeticum plants extract having potent while Leucas aspera plant extract shows the moderately defending property. Therefore, from the present study, it can be concluded that methanolic extract of selected indigenous plant extract possesses defending property. The observed defending activity of selected indigenous plant extracts against cyclophosphamide might be associated with active compounds and its antioxidant constituents such as poly-phenolic compound, flavonoids and other micronutrients....
Present study was aimed to evaluate the anti cancer activity of Annona squamosa leaves in selected cell lines in-vitro. Leaves of Annona squamosa were collected from local cultivating areas near to Anantapuramu, shade dried, mechanically made into powder. The powder was subjected to extraction by cold maceration method using water and alcohol mixture as solvent at a ratio of 3:2. The hydro alcoholic extract of Annona squamosa leaves was subjected to preliminary phytochemical screening using standard methods. In-vitro anticancer activity of hydro alcoholic extract of Annona squamosa leaves was performed on LNCap-FGC – Human carcinoma prostate, A549 – Human lung carcinoma and HEK-293 – Human kidney cell lines by MTT assay method at a concentration of 50, 100 and 200 microgram per ml using MTT assay. The percentage yield of Annona squamosa leaves using water: ethanol as solvent (3:2) was found to be 6.45% W/W. Preliminary phytochemical screening of hydro alcoholic extract of Annona squamosa leaves revealed the presence of Proteins, tannins, alkaloids and flavonoids. In anti cancer activity, the plant extract showed IC50 value of 200 mg/ml for all the three tested cell lines that is LNCap-FGC – Human carcinoma prostate, A549 – Human lung carcinoma and HEK-293 – Human kidney....
Ficus carica Linn. (Moraceae) commonly known as edible fig has been found to be associated with the several therapeutic properties. The current study used Ficus carica as an ameliorating agent against nephrotoxic effects of potassium dichromate. Potassium dichromate (K2Cr2O7) is a soluble hexavalent chromium compound that is used extensively in several industries. The rats were given pre-treatment of Ficus carica orally at a dose of (100 and 200 mg/kg body weight) for five consecutive days. Potassium dichromate 20 mg/kg b.wt was used as renal toxicant and injected subcutaneously in the neck region. The protective effects of Ficus carica on potassium dichromate induced nephrotoxicity was investigated by assaying oxidative stress biomarkers, lipid peroxidation, serum kidney toxicity markers and by histopathological examination of kidney. Ficus carica pre-treatment prevented deteriorative effects induced by potassium dichromate through a protective mechanism that involved reduction of increased oxidative stress and also by restoration of histopathological changes produced by the administration potassium dichromate....
The present investigation describes the phytochemical analysis and in-vitro antimicrobial property of Ficus amplissima Smith. bark extracts along with estimation of the inorganic components of the bark. The phytochemical analysis revealed that the major bioactive components of the bark extracts are alkaloids, phenolics, tannins, glycosides, saponins, proteins and steroids. The methanol extracts has high phenolic and flavonoids content than aqueous extracts. The antimicrobial data revealed that, the FAME showed significant antibacterial activity against P. aeruginosa, S. typhi and E. coli while FAAE showed significant antibacterial activity against B. subtilis extracts. FAME showed promising activity against A. flavus and FAAE showed activity against A. flavus....
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